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SCAEL Online Lab Notebook-Media
BG11 (Blue-Green Medium)
Freshwater algae and
protozoa
Stocks per litre
(1) NaNO3 15.0 g
per 500 ml
(2) K2HPO4 2.0 g
(3) MgSO4.7H2O 3.75 g
(4) CaCl2.2H2O 1.80 g
(5) Citric acid 0.30 g
(6) Ammonium ferric citrate green 0.30 g
(7) EDTANa2 0.05 g
(8) Na2CO3 1.00 g
(9) Trace metal solution per litre
H3BO3 2.86 g
MnCl2.4H2O 1.81 g
ZnSO4.7H2O 0.22 g
Na2MoO4.2H2O 0.39 g
CuSO4.5H2O 0.08 g
Co(NO3)2.6H2O 0.05 g
Medium Stock solution 1 100.0 ml
Stock solutions 2 - 8 10.0 ml each
Stock solution 9 1.0 ml
Make up
to 1 litre with deionized water. Adjust pH to
7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of
*Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
Stanier,
R.Y., Kunisawa, R., Mandel, M. & Cohen-Bazire, G. 1971
Purification and properties of
unicellular blue-green algae (Order Chroococcales).
Bacteriol. Rev. 35:
171-205.
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Algal Ecology Labs--331 Fort Johnson
Road--Charleston,
SC 29412.
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